User Manual Q&AAnswer
Apr 14, 2025 - 08:46 AM
The Attogene EL2047-01 Cylindrospermopsin ELISA Kit provides both semi-quantitative and quantitative results for the analysis of Cylindrospermopsin in water samples. Here is how you can interpret the results:
1. Semi-quantitative results: You can compare the absorbance (color intensity) of your sample to the absorbance of the calibrator wells. If your sample has lower absorbance (less color) than a calibrator well, it indicates a concentration of Cylindrospermopsin greater than the concentration of the calibrator. If your sample has higher absorbance (more color) than a calibrator well, it indicates a concentration of Cylindrospermopsin less than the concentration of the calibrator.
2. Quantitative results: To obtain quantitative results, you need to graph the absorbance (OD) from the calibrator wells (Y axis) versus the calibrator concentration (X axis). This can be done using a plate reader with software that uses either a 4-Parameter or Semi-log curve fit. If your plate reader software does not provide these curve fits, you can request a spreadsheet from Attogene that will perform the curve fit and sample concentration calculation.
It is important to note that if the absorbance of your sample is lower than the highest calibrator (2.0 ppb), the concentration of Cylindrospermopsin is too high and out of the range of the standard curve. In such cases, you should dilute the sample in laboratory grade water and rerun the assay. The results must then be multiplied by the dilution factor used.
Please refer to the provided manual for more detailed instructions and guidelines on result interpretation, including the expected range for the Cylindrospermopsin Positive Control (0.25 ppb).
Note: The information provided here is based on the manual for the Attogene EL2047-01 Cylindrospermopsin ELISA Kit. For complete and accurate interpretation of results, it is recommended to consult the manual and follow the instructions provided by Attogene.
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