User Manual Q&AAnswer
Mar 18, 2025 - 01:37 AM
To configure an analysis run using the DRAGEN TruSight Oncology 500 ctDNA Analysis Software on ICA, follow these steps:
1. Ensure that you have completed the prerequisites, which include purchasing ICA and enabling ICA Flow, uploading sequencing data files to an ICA project, and making sure a TSO 500 bundle is available for the project.
2. Launch the analysis in either graphical mode or command-line interface (CLI). In graphical mode, you need to configure the pipeline run settings, including selecting a TSO 500 project, TSO 500 pipeline, and the appropriate storage size. It is recommended to use the medium or large storage option.
3. If using the CLI, modify the same configuration fields as described in the graphical mode for each ICA CLI run.
4. The DRAGEN TSO 500 ctDNA Analysis Software v1.2 uses various tools to analyze sequencing data, including sample sheet validation, resource verification, run QC, FASTQ generation and validation, alignment, fusion calling, variant calling and filtering, annotation, TMB scoring, and more. For detailed information on each analysis method, refer to the DRAGEN TruSight Oncology 500 ctDNA Analysis Software v1.2 User Guide.
5. After the analysis is completed, the output folders will be generated. The analysis folder contains subfolders for results and logs/intermediates. The results folder contains aggregated metric and analytic files, while the logs/intermediates folder contains folders for each task in the pipeline, including log files, console output, error output, and intermediate results.
Please note that this answer provides a general overview of configuring an analysis run using the DRAGEN TruSight Oncology 500 ctDNA Analysis Software on ICA. For more detailed instructions and information, refer to the DRAGEN TruSight Oncology 500 ctDNA Analysis Software v1.2 User Guide (Document # 200017080) provided by Illumina.
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