User Manual Q&AAnswer
Feb 28, 2025 - 02:33 AM
To prepare the Incubation mix for the Olink Target 48 High Multiplex Immunoassay Panels, follow these steps:
1. Vortex and spin down the Incubation mix.
2. Transfer 23 µL of the Incubation mix to each well of a new 8-well strip.
3. Transfer 3 μL of Incubation mix to each well of the first 6 columns of a 96-well plate by reverse pipetting and name the plate Incubation Plate.
4. Add 1 µL of each sample using a multi-channel pipette to the bottom of the well, 1 µl of Sample Control to the three top wells (yellow), 1 µL of Negative Control to two wells (red), and 1 µL of Calibrators to three wells (green), according to the plate layout.
5. Seal the plate with an adhesive plastic film, spin at 400 – 1000 x g, 1 min at room temperature.
6. Incubate overnight at +4 °C.
Please refer to the Olink Target 48 High Multiplex Immunoassay Panels Instructions for more detailed information and specific guidelines.
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