Answer
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Feb 26, 2025 - 03:10 AM
To plate the master mix for the Co-Dx COVID-R-002 Logix Smart SARS-CoV-2 RUO product, follow these steps:
1. Load the Co-Dx Box software.
2. In the Clean area, clean work surfaces with water > 10% bleach > water > 70% alcohol (in that order), or another equivalent method of cleaning that disinfects and degrades nucleic acids.
3. Thaw the master mix (MM) and the nuclease-free water (NFW) (for the no-template control [NC]), on ice.
4. Briefly vortex and centrifuge the MM and NFW reagents.
5. Add 5 µL of MM into every well that will be used (1 well for NC, 1 well for positive control [PC], and 1 well for each sample).
6. Pipet 5 µL of NFW into only the NC well and cap the NC well.
7. Place the reagents back into the freezer.
Please note that these instructions are a summary and should be followed in conjunction with the Co-Dx COVID-R-002 Logix Smart SARS-CoV-2 RUO Product User Guide for complete and accurate information.
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